Journal: Scientific Reports

Article Title: Thyroid hormone status defines brown adipose tissue activity and browning of white adipose tissues in mice

doi: 10.1038/srep38124

Figure Lengend Snippet: ( A ) Representative Ucp1 immunohistochemical stainings in gWAT and iWAT in eu-, hyper- and hypothyroid mice; ( B ) ex-vivo 14C-palmitate uptake in n = 10 mice/group. ( C ) Ardb1 and Ardb3 mRNA expression in gWAT and iWAT (n = 5 mice/group). Data are presented as mean and SEM (*p < 0.05; **p < 0.01).

Article Snippet: The following antibodies and dilutions were used: mouse polyclonal anti-Ucp1 antibody (1:500; Merck Millipore; Darmstadt, Germany), pHSL and HSL(1:1000; Cell Signaling Technology, Danvers, USA).

Techniques: Immunohistochemical staining, Ex Vivo, Expressing

Journal: Scientific Reports

Article Title: Thyroid hormone status defines brown adipose tissue activity and browning of white adipose tissues in mice

doi: 10.1038/srep38124

Figure Lengend Snippet: ( A ) Representative hematoxylin and eosin sections; ( B ) Lipid droplet size and number (n = 10/group); ( C ) Expression of genes associated with thermogenesis, lipolysis, recruitment and β-adrenergic signaling (n = 7–10/group); ( D ) Quantification of BAT UCP1, pHSL and HSL protein by Western Blot analysis and normalization against β-actin as loading control (n = 4 mice/group). Data are presented as mean and SEM (*p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: The following antibodies and dilutions were used: mouse polyclonal anti-Ucp1 antibody (1:500; Merck Millipore; Darmstadt, Germany), pHSL and HSL(1:1000; Cell Signaling Technology, Danvers, USA).

Techniques: Expressing, Western Blot, Control